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I am using: K2 » Latest items from (one or more) users or categories » Latest items from (one or more) users or categories to show the latest 1 item from 3 different categories on the Home Page. I suspect it's because 2 columns have a full width image and the other is a floated image with text, but, if you look at the 2 spcaings, the one between the left and middle columns is obviously smaller than the space between the middle and right columns.

The image width that is being used is not covering all of the available space so this why the spacing does not appear equal.